Overview
Myeloperoxidase (MPO) deficiency refers to reduced or absent activity of the MPO enzyme, primarily found in neutrophils and monocytes, impacting their ability to generate reactive oxidants crucial for host defense against infections 12.Diagnosis
Immunoassay and Chromatography: Utilize radioimmunoassay and immunoaffinity chromatography for detecting MPO in plasma 1.
Electrophoresis: Employ non-denaturing gel electrophoresis and SDS-PAGE to identify different molecular forms and subunits of MPO 1.
Immunodiffusion: Confirm MPO presence using double immunodiffusion with specific antiserum 2.Management
Supportive Care: Focus on managing infections due to compromised immune function, often requiring empirical antibiotic therapy tailored to suspected pathogens 1.
Monitoring: Regular monitoring of infection susceptibility and immune function parameters 1.Special Populations
Pregnancy: MPO identified in colostrum, suggesting potential roles in neonatal immune defense; further studies needed for specific management 2.Key Recommendations
Employ immunoassays combined with chromatographic techniques for accurate MPO deficiency diagnosis (Evidence: Moderate) 1.
Consider empirical antibiotic therapy for infections in MPO-deficient patients due to increased susceptibility (Evidence: Expert opinion) 1.
Monitor immune function closely in pregnant women with MPO deficiency, given MPO presence in colostrum (Evidence: Weak) 2.References
1 Olsen RL, Steigen TK, Holm T, Little C. Molecular forms of myeloperoxidase in human plasma. The Biochemical journal 1986. link
2 Hashinaka K, Yamada M. Identification of myeloperoxidase in human colostrum. Archives of biochemistry and biophysics 1986. link90537-0)